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This study was conducted to optimize medium compositions and culture conditions on plant regeneration through organogenesis from leaf explant in ´Kyoho´ grape (Vitis labrusca × V. vinifera). Adventitious shoot formation from in vitro cultured leaf segments was affected by basal medium, plant growth regulator and light condition. Within the first 7 days of culture on shoot regeneration medium, the petiolar stub of explants was swollen and formed callus. Adventitious structures formed on the petiolar stub after 14 days. Among plant growth regulator treatment, 2.0 ㎎·L^(-1) BA and 0.01 ㎎·L^(-1) IBA produced the highest shoot regeneration frequency. Adventitious shoots were regenerated effectively on the modified MS medium such as 1/2TMS and 1/2MMS. Among three gelling agents, agar showed higher shoot regeneration than agarose and Phytagel. The dark incubation for 3-4 weeks at the initial culture periods was effective for shoot regeneration. In conclusion the most excellent shoot regeneration from leaf explant was obtained with dark incubation of initial 3 weeks on 1/2TMS medium with 2.0 ㎎·L^(-1) BA, 0.01 ㎎·L^(-1) IBA and 8 g·L^(-1) agar, in which the regeneration frequency reached up to 80%. The regenerated shoots rooted successfully in MS medium with 0.1 ㎎·L^(-1) IAA, and plantlets were acclimated.
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